ARI-TOX Marine Wildlife CellBank

Cell line ID: DD03s-p
Download cell line details as a PDF file

Animal Group: Mammals
Sub-Group: Dugong
Species Name: Dugong (Dugong dugon)

Tissue: Skin
Cell type: Fibroblast

Cell culture source
Sex: F
Age (or age-class): I
Length (cm): 215.5
Weight (kg): N/A
Condition: Healthy
Location sampled (GPS co-ordinates): Moreton Bay (-27.279373, 153.344700)
Date of sample collection: 03/11/2018

Cell culture instructions
Growth medium: DMEM + GlutaMax with 10% fetal bovine serum (FBS). Antibiotics can also be used at concentrations of 1% penicillin/streptomycin (10,000 U/mL stock), 1% amphotericin B (250 µg/mL stock)
Incubation temperature (°C): 37
Incubation CO2 (%): 5%

Cryopreservation: All aliquots are at a concentration 1,000,000 cells/mL in 1 mL of growth medium (see care instructions) supplemented with 10% dimethyl sulfoxide (DMSO)

DNA sequence: Dugong dugon, 99.39%

Karyotype: N/A

Mycoplasma contamination: No

Notes: Nil