ARI-TOX Marine Wildlife CellBank

Cell line ID: CB02s-p
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Animal Group: Mammals
Sub-Group: Dolphins
Species Name: Common bottlenose dolphin (Tursiops truncatus)

Tissue: Skin
Cell type: Fibroblast

Cell culture source
Sex: M
Age (or age-class): ~43
Length (cm): N/A
Weight (kg): N/A
Condition: Wild caught 1985
Location sampled (GPS co-ordinates): Sea World (-27.960236, 153.425884)
Date of sample collection: 22/03/2017

Cell culture instructions: Growth medium: DMEM + GlutaMax with 10% fetal bovine serum (FBS). Antibiotics can also be used at concentrations of 1% penicillin/streptomycin (10,000 U/mL stock), 1% amphotericin B (250 µg/mL stock)
Incubation temperature (°C): 37
Incubation CO2 (%): 5%

Cryopreservation: All aliquots are at a concentration 1,000,000 cells/mL in 1 mL of growth medium (see care instructions) supplemented with 10% dimethyl sulfoxide (DMSO)

DNA sequence: Tursiops truncatus, 99.85%

Karyotype: 44, XY

Mycoplasma contamination: Inconclusive

Notes: Nil