ARI-TOX Marine Wildlife CellBank

Cell line ID: GT06s-p
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Animal Group: Reptiles
Sub-Group: Sea turtles
Species Name: Green turtle (Chelonia mydas)

Tissue: Skin
Cell type: Fibroblast

Cell culture source
Sex: I
Age (or age-class): SA
Length (cm): 65
Weight (kg): N/A
Condition: Healthy
Location sampled (GPS co-ordinates): Booral (-25.364905, 152.916894)
Date of sample collection: 21/11/2015

Cell culture instructions
Growth medium: RPMI-1640 with 10% fetal bovine serum (FBS). Antibiotics can also be used at concentrations of 1% penicillin/streptomycin (10,000 U/mL stock), 1% amphotericin B (250 µg/mL stock)
Incubation temperature (°C): 30
Incubation CO2 (%): 5%

Cryopreservation
All aliquots are at a concentration 1,000,000 cells/mL in 1 mL of growth medium (see care instructions) supplemented with 10% dimethyl sulfoxide (DMSO)

DNA sequence
Chelonia mydas, 100%, two peaks – mixed, aligns with Caretta caretta but not 100%

Karyotype: 56

Mycoplasma contamination: No

Notes: Nil