ARI-TOX Marine Wildlife CellBank

Cell line ID: RD01g-p
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Animal Group: Mammals
Sub-Group: Dolphins
Species Name: Risso’s dolphin (Grampus griseus)

Tissue: Lung
Cell type: Fibroblast

Cell culture source
Sex: N/A
Age (or age-class): N/A
Length (cm): N/A
Weight (kg): N/A
Condition: Stranded
Location sampled (GPS co-ordinates): Dolphin Marine Magic (-30.298648, 153.136188)
Date of sample collection: 2016

Cell culture instructions: Growth medium: DMEM + GlutaMax with 10% fetal bovine serum (FBS). Antibiotics can also be used at concentrations of 1% penicillin/streptomycin (10,000 U/mL stock), 1% amphotericin B (250 µg/mL stock)
Incubation temperature (°C): 37
Incubation CO2 (%): 5%

Cryopreservation: All aliquots are at a concentration 1,000,000 cells/mL in 1 mL of growth medium (see care instructions) supplemented with 10% dimethyl sulfoxide (DMSO)

DNA sequence: Grampus griseus 99.85%

Karyotype: 44, XX

Mycoplasma contamination: No

Notes: Nil